Ontology highlight
ABSTRACT:
SUBMITTER: Jin F
PROVIDER: S-EPMC2803036 | biostudies-literature | 2006 Mar
REPOSITORIES: biostudies-literature
Jin Fulai F Hazbun Tony T Michaud Gregory A GA Salcius Michael M Predki Paul F PF Fields Stanley S Huang Jing J
Nature methods 20060301 3
The generation of large-scale data sets is a fundamental requirement of systems biology. But despite recent advances, generation of such high-coverage data remains a major challenge. We developed a pooling-deconvolution strategy that can dramatically decrease the effort required. This strategy, pooling with imaginary tags followed by deconvolution (PI-deconvolution), allows the screening of 2(n) probe proteins (baits) in 2 x n pools, with n replicates for each bait. Deconvolution of baits with t ...[more]