Unknown

Dataset Information

0

Brain region-specific N-glycosylation and lipid rafts association of the rat mu opioid receptor.

ABSTRACT: The mu opioid receptor (MOR) in the rat and mouse caudate putamen (CPu) and thalamus was demonstrated as diffuse and broad bands by Western blot with a polyclonal antibody against a C-terminal peptide of MOR, which were absent in the cerebellum and brains of MOR-knockout mice. The electrophoretic mobility of MOR differed in the two brain regions with median relative molecular masses (Mr's) of 75 kDa (CPu) vs. 66 kDa (thalamus) for the rat, and 74 kDa (CPu) vs. 63 kDa (thalamus) for the mouse, which was due to its differential N-glycosylation. Rat MOR in CPu was found mainly associated with low-density cholesterol- and ganglioside M1 (GM1)-enriched membrane subdomains (lipid rafts), while the MOR in the thalamus was present in rafts and non-rafts without preference. Cholesterol reduction by methyl-beta-cyclodextrin decreased DAGMO-induced [35S]GTPgammaS binding in rat CPu membranes to a greater extent than in the thalamus membranes.

SUBMITTER: Huang P 

PROVIDER: S-EPMC2806241 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Brain region-specific N-glycosylation and lipid rafts association of the rat mu opioid receptor.

Huang Peng P   Chen Chongguang C   Xu Wei W   Yoon Su-In SI   Unterwald Ellen M EM   Pintar John E JE   Wang Yulin Y   Chong Parkson Lee-Gau PL   Liu-Chen Lee-Yuan LY  

Biochemical and biophysical research communications 20071031 1

The mu opioid receptor (MOR) in the rat and mouse caudate putamen (CPu) and thalamus was demonstrated as diffuse and broad bands by Western blot with a polyclonal antibody against a C-terminal peptide of MOR, which were absent in the cerebellum and brains of MOR-knockout mice. The electrophoretic mobility of MOR differed in the two brain regions with median relative molecular masses (Mr's) of 75 kDa (CPu) vs. 66 kDa (thalamus) for the rat, and 74 kDa (CPu) vs. 63 kDa (thalamus) for the mouse, wh  ...[more]

Similar Datasets

Unknown Agonist treatment did not affect association of mu opioid receptors with lipid rafts and cholesterol reduction had opposite effects on the receptor-mediated signaling in rat brain and CHO cells.
| S-EPMC3715317 | biostudies-literature
Unknown Region-specific transcriptional response to chronic nicotine in rat brain.
| S-EPMC3098570 | biostudies-literature
Unknown Prion protein accumulation in lipid rafts of mouse aging brain.
| S-EPMC3769255 | biostudies-literature
Unknown Association of rat8 with Fyn protein kinase via lipid rafts is required for rat mammary cell differentiation in vitro.
| S-EPMC357021 | biostudies-literature
Unknown Brain region-specific gene expression profiles in freshly isolated rat microglia.
| S-EPMC4357261 | biostudies-literature
Unknown Cholesterol regulates micro-opioid receptor-induced beta-arrestin 2 translocation to membrane lipid rafts.
| S-EPMC3127540 | biostudies-literature
Unknown CD317 Activates EGFR by Regulating Its Association with Lipid Rafts.
| S-EPMC6540786 | biostudies-literature
Unknown The untranslated region of (mu)-opioid receptor mRNA contributes to reduced opioid sensitivity in CXBK mice.
| S-EPMC6762225 | biostudies-literature
Unknown The role of N53Q mutation on the rat mu-opioid receptor function.
| S-EPMC2884312 | biostudies-literature
Unknown Morphine-induced internalization of the L83I mutant of the rat μ-opioid receptor.
| S-EPMC4292971 | biostudies-literature