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Acquisition of chemiluminescent signals from immunoblots with a digital single-lens reflex camera.

ABSTRACT: We found that certain mid-range consumer-level digital single-lens reflex (SLR) cameras using full-frame complementary metal oxide semiconductor (CMOS) sensors outperform X-ray film in acquiring signals from immunoblots that use enhanced chemiluminescence for detection. These cameras exhibit a sensitivity comparable to X-ray film, yet they provide a 3-fold increase in linear dynamic range and substantial cost savings over time, are more convenient to use, and eliminate the chemical waste associated with processing film.

SUBMITTER: Khoury MK

PROVIDER: S-EPMC2808431 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Acquisition of chemiluminescent signals from immunoblots with a digital single-lens reflex camera.

Khoury Mitri K MK Parker Ian I Aswad Dana W DW

Analytical biochemistry 20090927 1

We found that certain mid-range consumer-level digital single-lens reflex (SLR) cameras using full-frame complementary metal oxide semiconductor (CMOS) sensors outperform X-ray film in acquiring signals from immunoblots that use enhanced chemiluminescence for detection. These cameras exhibit a sensitivity comparable to X-ray film, yet they provide a 3-fold increase in linear dynamic range and substantial cost savings over time, are more convenient to use, and eliminate the chemical waste associa ...[more]

PMID: 19788886

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