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Direct link between toluene degradation in contaminated-site microcosms and a Polaromonas strain.

ABSTRACT: Stable isotope probing (SIP) was used to identify the aerobic toluene-degrading microorganism in soil microcosms. Several approaches (terminal restriction fragment length polymorphism, 16S rRNA gene sequencing, and quantitative PCR) provided evidence that the microorganism responsible was a member of the genus Polaromonas and could grow on toluene. This microorganism also transformed benzene, but not m-xylene or cis-dichloroethene.

SUBMITTER: Sun W

PROVIDER: S-EPMC2813006 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Direct link between toluene degradation in contaminated-site microcosms and a Polaromonas strain.

Sun Weimin W Xie Shuguang S Luo Chunling C Cupples Alison M AM

Applied and environmental microbiology 20091211 3

Stable isotope probing (SIP) was used to identify the aerobic toluene-degrading microorganism in soil microcosms. Several approaches (terminal restriction fragment length polymorphism, 16S rRNA gene sequencing, and quantitative PCR) provided evidence that the microorganism responsible was a member of the genus Polaromonas and could grow on toluene. This microorganism also transformed benzene, but not m-xylene or cis-dichloroethene. ...[more]

PMID: 20008173

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