Dataset Information

Confirmation of direct interaction between H3K56cr and GLYR1 by H3K*56cr Site-Link in living cells

ABSTRACT: As we have found that H3K56cr can crosslink with GLYR1 in living cells, we now perform H3K*cr Site-Link in GLYR1 overexpressed cells. The subsequent 365-nm light irradiation could activate the diazirine and covalently capture potential effector proteins on native chromatin in living cells. The crosslinked band could be identified after gel-based proteomics and pLink assisted MS/MS analysis of the crosslinked peptide.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Early Embryonic Cell

SUBMITTER: Fangfei Qin

LAB HEAD: Peng R. Chen

PROVIDER: PXD033960 | Pride | 2023-03-06

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	GLYR1-H3K56cr.raw	Raw
	GLYR1-H3K56cr_plink.xlsx	Xlsx
	checksum.txt	Txt

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Publications

Linking chromatin acylation mark-defined proteome and genome in living cells.

Qin Fangfei F Li Boyuan B Wang Hui H Ma Sihui S Li Jiaofeng J Liu Shanglin S Kong Linghao L Zheng Huangtao H Zhu Rongfeng R Han Yu Y Yang Mingdong M Li Kai K Ji Xiong X Chen Peng R PR

Cell 20230301 5

A generalizable strategy with programmable site specificity for in situ profiling of histone modifications on unperturbed chromatin remains highly desirable but challenging. We herein developed a single-site-resolved multi-omics (SiTomics) strategy for systematic mapping of dynamic modifications and subsequent profiling of chromatinized proteome and genome defined by specific chromatin acylations in living cells. By leveraging the genetic code expansion strategy, our SiTomics toolkit revealed di ...[more]

PMID: 36868209

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