Use of green fluorescent protein to monitor cell envelope stress in Lactococcus lactis.
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ABSTRACT:
SUBMITTER: Campelo AB
PROVIDER: S-EPMC2813010 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Publications
Use of green fluorescent protein to monitor cell envelope stress in Lactococcus lactis.
Campelo Ana Belén AB Rodríguez Ana A Martínez Beatriz B
Applied and environmental microbiology 20091130 3
A Lactococcus lactis reporter system suitable to detect cell envelope stress in high-throughput settings was developed by fusing the CesR-regulated promoter of llmg0169 to the gfp(uv) gene. A dot blot assay allowed fast detection of green fluorescent protein (GFP) fluorescence even at low production levels. Unexpectedly, this promoter was also induced by mitomycin C via CesR. ...[more]
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