Ontology highlight
ABSTRACT:
SUBMITTER: Overkamp W
PROVIDER: S-EPMC3811213 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Applied and environmental microbiology 20130816 20
Green fluorescent protein (GFP) offers efficient ways of visualizing promoter activity and protein localization in vivo, and many different variants are currently available to study bacterial cell biology. Which of these variants is best suited for a certain bacterial strain, goal, or experimental condition is not clear. Here, we have designed and constructed two "superfolder" GFPs with codon adaptation specifically for Bacillus subtilis and Streptococcus pneumoniae and have benchmarked them aga ...[more]