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Phenomenological partial-specific volumes for G-quadruplex DNAs.

ABSTRACT: Accurate partial-specific volume ([Formula: see text]) values are required for sedimentation velocity and sedimentation equilibrium analyses. For nucleic acids, the estimation of these values is complicated by the fact that [Formula: see text] depends on base composition, secondary structure, solvation and the concentrations and identities of ions in the surrounding buffer. Here we describe sedimentation equilibrium measurements of the apparent isopotential partial-specific volume /' for two G-quadruplex DNAs and a single-stranded DNA of similar molecular weight and base composition. The G-quadruplex DNAs are a 22 nucleotide fragment of the human telomere consensus sequence and a 27 nucleotide fragment from the human c-myc promoter. The single-stranded DNA is 26 nucleotides long and is designed to have low propensity to form secondary structures. Parallel measurements were made in buffers containing NaCl and in buffers containing KCl, spanning the range 0.09 M

SUBMITTER: Hellman LM

PROVIDER: S-EPMC2813399 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Phenomenological partial-specific volumes for G-quadruplex DNAs.

Hellman Lance M LM Rodgers David W DW Fried Michael Gregory MG

European biophysics journal : EBJ 20090224 3

Accurate partial-specific volume ([Formula: see text]) values are required for sedimentation velocity and sedimentation equilibrium analyses. For nucleic acids, the estimation of these values is complicated by the fact that [Formula: see text] depends on base composition, secondary structure, solvation and the concentrations and identities of ions in the surrounding buffer. Here we describe sedimentation equilibrium measurements of the apparent isopotential partial-specific volume /' for two G-q ...[more]

PMID: 19238377

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