Ontology highlight
ABSTRACT:
SUBMITTER: Jawerth LM
PROVIDER: S-EPMC2814201 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Biophysical journal 20100201 3
We investigate the dependence of fiber brightness on three-dimensional fiber orientation when imaging biopolymer networks with confocal reflection microscopy (CRM) and confocal fluorescence microscopy (CFM). We compare image data of fluorescently labeled type I collagen networks concurrently acquired using each imaging modality. For CRM, fiber brightness decreases for more vertically oriented fibers, leaving fibers above approximately 50 degrees from the imaging plane entirely undetected. As a r ...[more]