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Polarization microscopy for characterizing fiber orientation of ocular tissues.

ABSTRACT: Characterizing the collagen fiber orientation and organization in the eye is necessary for a complete understanding of ocular biomechanics. In this study, we assess the performance of polarized light microscopy to determine collagen fiber orientation of ocular tissues. Our results demonstrate that the method provides objective, accurate, repeatable and robust data on fiber orientation with µm-scale resolution over a broad, cm-scale, field of view, unaffected by formalin fixation, without requiring tissue dehydration, labeling or staining. Together, this shows that polarized light microscopy is a powerful method for studying collagen architecture in the eye, with applications ranging from normal physiology and aging, to pathology and transplantation.

SUBMITTER: Jan NJ

PROVIDER: S-EPMC4679248 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Polarization microscopy for characterizing fiber orientation of ocular tissues.

Jan Ning-Jiun NJ Grimm Jonathan L JL Tran Huong H Lathrop Kira L KL Wollstein Gadi G Bilonick Richard A RA Ishikawa Hiroshi H Kagemann Larry L Schuman Joel S JS Sigal Ian A IA

Biomedical optics express 20151105 12

Characterizing the collagen fiber orientation and organization in the eye is necessary for a complete understanding of ocular biomechanics. In this study, we assess the performance of polarized light microscopy to determine collagen fiber orientation of ocular tissues. Our results demonstrate that the method provides objective, accurate, repeatable and robust data on fiber orientation with µm-scale resolution over a broad, cm-scale, field of view, unaffected by formalin fixation, without requiri ...[more]

PMID: 26713188

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