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Cationic nucleoside lipids based on a 3-nitropyrrole universal base for siRNA delivery.

ABSTRACT: Cationic nucleoside lipids based on a 3-nitropyrrole universal base were prepared from D-ribose using a straightforward chemical synthesis. Several studies including DLS, TEM, and ethidium bromide (EthBr) assay demonstrated that these amphiphilic molecules form supramolecular organizations of nanometer size in aqueous solutions and are able to bind nucleic acids. siRNA knockdown experiments were performed with these nucleolipids, and we observed protein knockdown activity similar to the siPORT NeoFX positive control. No significant cytotoxicity was found.

SUBMITTER: Ceballos C

PROVIDER: S-EPMC2819530 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

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Cationic nucleoside lipids based on a 3-nitropyrrole universal base for siRNA delivery.

Ceballos Claire C Prata Carla A H CA Giorgio Suzanne S Garzino Frédéric F Payet Dominique D Barthélémy Philippe P Grinstaff Mark W MW Camplo Michel M

Bioconjugate chemistry 20090201 2

Cationic nucleoside lipids based on a 3-nitropyrrole universal base were prepared from D-ribose using a straightforward chemical synthesis. Several studies including DLS, TEM, and ethidium bromide (EthBr) assay demonstrated that these amphiphilic molecules form supramolecular organizations of nanometer size in aqueous solutions and are able to bind nucleic acids. siRNA knockdown experiments were performed with these nucleolipids, and we observed protein knockdown activity similar to the siPORT N ...[more]

PMID: 19159294

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