Stability of murine cytomegalovirus genome after in vitro and in vivo passage.
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ABSTRACT:
SUBMITTER: Cheng TP
PROVIDER: S-EPMC2820925 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Publications
Stability of murine cytomegalovirus genome after in vitro and in vivo passage.
Cheng Tammy P TP Valentine Mark C MC Gao Jian J Pingel Jeanette T JT Yokoyama Wayne M WM
Journal of virology 20091216 5
While large DNA viruses are thought to have low mutation rates, only a small fraction of their genomes have been analyzed at the single-nucleotide level. Here, we defined the genetic stability of murine cytomegalovirus (MCMV) by whole-genome sequencing. Independently assembled sequences of three sister plaques showed only two single-base-pair substitutions after in vitro passage. In vivo-passaged MCMV likewise demonstrated low mutation rates, comparable to those after in vitro passage, indicatin ...[more]
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