Ontology highlight
ABSTRACT:
SUBMITTER: Hirano M
PROVIDER: S-EPMC2823519 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Hirano Minako M Takeuchi Yuko Y Aoki Takaaki T Yanagida Toshio T Ide Toru T
The Journal of biological chemistry 20091203 6
A change of cytosolic pH 7 to 4 opens the bacterial potassium channel KcsA. However, the overall gating mechanism leading to channel opening, especially the contribution of the cytoplasmic domain, remains unsolved. Here we report that deletion of the cytoplasmic domain resulted in changes in channel conductance and gating behavior at pH 4 without channel opening at pH 7. To probe for rearrangements in the cytoplasmic domain during channel opening, amino acid residues were substituted with cystei ...[more]