Ontology highlight
ABSTRACT:
SUBMITTER: Picotti P
PROVIDER: S-EPMC2825542 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
Picotti Paola P Bodenmiller Bernd B Mueller Lukas N LN Domon Bruno B Aebersold Ruedi R
Cell 20090806 4
The rise of systems biology implied a growing demand for highly sensitive techniques for the fast and consistent detection and quantification of target sets of proteins across multiple samples. This is only partly achieved by classical mass spectrometry or affinity-based methods. We applied a targeted proteomics approach based on selected reaction monitoring (SRM) to detect and quantify proteins expressed to a concentration below 50 copies/cell in total S. cerevisiae digests. The detection range ...[more]