Ontology highlight
ABSTRACT:
SUBMITTER: Mohrs K
PROVIDER: S-EPMC2826320 | biostudies-literature | 2005 Oct
REPOSITORIES: biostudies-literature
Mohrs Katja K Wakil Adil E AE Killeen Nigel N Locksley Richard M RM Mohrs Markus M
Immunity 20051001 4
To monitor IL-4 expression at the single-cell level, we generated mice with insertions of different reporter genes into both copies of the Il4 gene that permitted the simultaneous analysis of IL-4 transcripts via GFP and IL-4 protein secretion by use of huCD2. Innate and adaptive cells competent for IL-4 production were marked by GFP, while cells that presently or recently secreted IL-4 additionally displayed huCD2. After challenge with the strictly enteric helminth, Heligmosomoides polygyrus, G ...[more]