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Biophysical characterization reveals structural disorder in the developmental transcriptional regulator LBH.

ABSTRACT: Limb-bud and heart (LBH) is a key transcriptional regulator in vertebrates with pivotal roles in embryonic development and human disease. Herein, using a diverse array of biophysical techniques, we report the first structural characterization of LBH pertinent to its biological function. Our data reveal that LBH is structurally disordered with no discernable secondary or tertiary structure and exudes rod-like properties in solution. Consistent with these observations, we also demonstrate that LBH is conformationally flexible and thus may be capable of adapting distinct conformations under specific physiological contexts. We propose that LBH is a member of the intrinsically disordered protein (IDP) family, and that conformational plasticity may play a significant role in modulating LBH-dependent transcriptional processes.

SUBMITTER: Al-Ali H

PROVIDER: S-EPMC2827303 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature

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Biophysical characterization reveals structural disorder in the developmental transcriptional regulator LBH.

Al-Ali Hassan H Rieger Megan E ME Seldeen Kenneth L KL Harris Thomas K TK Farooq Amjad A Briegel Karoline J KJ

Biochemical and biophysical research communications 20091211 1

Limb-bud and heart (LBH) is a key transcriptional regulator in vertebrates with pivotal roles in embryonic development and human disease. Herein, using a diverse array of biophysical techniques, we report the first structural characterization of LBH pertinent to its biological function. Our data reveal that LBH is structurally disordered with no discernable secondary or tertiary structure and exudes rod-like properties in solution. Consistent with these observations, we also demonstrate that LBH ...[more]

PMID: 20005203

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