Ontology highlight
ABSTRACT:
SUBMITTER: Simmer F
PROVIDER: S-EPMC2828748 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Simmer Femke F Buscaino Alessia A Kos-Braun Isabelle C IC Kagansky Alexander A Boukaba Abdelhalim A Urano Takeshi T Kerr Alastair R W AR Allshire Robin C RC
EMBO reports 20100108 2
RNA interference (RNAi) is widespread in eukaryotes and regulates gene expression transcriptionally or post-transcriptionally. In fission yeast, RNAi is tightly coupled to template transcription and chromatin modifications that establish heterochromatin in cis. Exogenous double-stranded RNA (dsRNA) triggers seem to induce heterochromatin formation in trans only when certain silencing proteins are overexpressed. Here, we show that green fluorescent protein (GFP) hairpin dsRNA allows production of ...[more]