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Transfer-messenger RNA controls the translation of cell-cycle and stress proteins in Streptomyces.


ABSTRACT: The transfer-messenger RNA (tmRNA)-mediated trans-translation mechanism is highly conserved in bacteria and functions primarily as a system for the rescue of stalled ribosomes and the removal of aberrantly produced proteins. Here, we show that in the antibiotic-producing soil bacterium Streptomyces coelicolor, trans-translation has a specialized role in stress management. Analysis of proteins that were carboxy-terminally His(8)-tagged by a recombinant tmRNA identified only 10 targets, including the stress proteins: DnaK heat-shock protein 70, thiostrepton-induced protein A, universal stress protein A, elongation factor Tu3, and the cell-cycle control proteins DasR, SsgA, SsgF and SsgR. Although tmRNA-tagged proteins are degraded swiftly, the translation of dnaK and dasR messenger RNAs (mRNAs) depends fully on tmRNA, whereas transcription is unaffected. The data unveil a surprisingly dedicated functionality for tmRNA, promoting the translation of the same mRNA it targets, at the expense of sacrificing the first nascent protein. In streptomycetes, tmRNA has evolved into a dedicated task force that ensures the instantaneous response to the exposure to stress.

SUBMITTER: Barends S 

PROVIDER: S-EPMC2828758 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Transfer-messenger RNA controls the translation of cell-cycle and stress proteins in Streptomyces.

Barends Sharief S   Zehl Martin M   Bialek Sylwia S   de Waal Ellen E   Traag Bjørn A BA   Willemse Joost J   Jensen Ole Nørregaard ON   Vijgenboom Erik E   van Wezel Gilles P GP  

EMBO reports 20091218 2


The transfer-messenger RNA (tmRNA)-mediated trans-translation mechanism is highly conserved in bacteria and functions primarily as a system for the rescue of stalled ribosomes and the removal of aberrantly produced proteins. Here, we show that in the antibiotic-producing soil bacterium Streptomyces coelicolor, trans-translation has a specialized role in stress management. Analysis of proteins that were carboxy-terminally His(8)-tagged by a recombinant tmRNA identified only 10 targets, including  ...[more]

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