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Medium weight neurofilament mRNA in goldfish Mauthner axoplasm.

ABSTRACT: Although axons are generally considered to lack the ability to synthesize proteins, the Mauthner axon (M-axon) of the goldfish has been reported to contain some of the basic components of the translational machinery, such as transfer RNA (tRNA), ribosomal RNA (rRNA), and ribosomes. To determine if the M-axon also contains mRNA, we isolated samples of M-axoplasm free of glial contamination as demonstrated by the absence of glial-specific mRNA and protein. Reverse transcription-polymerase chain reaction (RT-PCR) of M-axoplasmic cDNA in the presence of primers for the goldfish medium-weight neurofilament (NF-M) gene produced a single product of the expected length for RT-PCR amplification of goldfish NF-M mRNA. This mRNA might direct protein synthesis of NF-M within the M-axoplasm.

SUBMITTER: Weiner OD

PROVIDER: S-EPMC2830807 | biostudies-literature | 1996 Aug

REPOSITORIES: biostudies-literature

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Medium weight neurofilament mRNA in goldfish Mauthner axoplasm.

Weiner O D OD Zorn A M AM Krieg P A PA Bittner G D GD

Neuroscience letters 19960801 2

Although axons are generally considered to lack the ability to synthesize proteins, the Mauthner axon (M-axon) of the goldfish has been reported to contain some of the basic components of the translational machinery, such as transfer RNA (tRNA), ribosomal RNA (rRNA), and ribosomes. To determine if the M-axon also contains mRNA, we isolated samples of M-axoplasm free of glial contamination as demonstrated by the absence of glial-specific mRNA and protein. Reverse transcription-polymerase chain re ...[more]

PMID: 8858614

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