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16S rRNA gene sequencing in routine identification of anaerobic bacteria isolated from blood cultures.


ABSTRACT: A comparison between conventional identification and 16S rRNA gene sequencing of anaerobic bacteria isolated from blood cultures in a routine setting was performed (n = 127). With sequencing, 89% were identified to the species level, versus 52% with conventional identification. The times for identification were 1.5 days and 2.8 days, respectively.

SUBMITTER: Justesen US 

PROVIDER: S-EPMC2832449 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature

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16S rRNA gene sequencing in routine identification of anaerobic bacteria isolated from blood cultures.

Justesen Ulrik Stenz US   Skov Marianne Nielsine MN   Knudsen Elisa E   Holt Hanne Marie HM   Søgaard Per P   Justesen Tage T  

Journal of clinical microbiology 20100113 3


A comparison between conventional identification and 16S rRNA gene sequencing of anaerobic bacteria isolated from blood cultures in a routine setting was performed (n = 127). With sequencing, 89% were identified to the species level, versus 52% with conventional identification. The times for identification were 1.5 days and 2.8 days, respectively. ...[more]

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