De novo species identification using 16S rRNA gene nanopore sequencing.
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ABSTRACT:
SUBMITTER: Angell IL
PROVIDER: S-EPMC7585375 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Publications
De novo species identification using 16S rRNA gene nanopore sequencing.
Angell Inga Leena IL Nilsen Morten M Carlsen Karin C Lødrup KCL Carlsen Kai-Håkon KH Hedlin Gunilla G Jonassen Christine M CM Marsland Benjamin B Nordlund Björn B Rehbinder Eva Maria EM Saunders Carina C Skjerven Håvard Ove HO Staff Anne Cathrine AC Söderhäll Cilla C Vettukattil Riyas R Rudi Knut K
PeerJ 20201021
Nanopore sequencing is rapidly becoming more popular for use in various microbiota-based applications. Major limitations of current approaches are that they do not enable de novo species identification and that they cannot be used to verify species assignments. This severely limits applicability of the nanopore sequencing technology in taxonomic applications. Here, we demonstrate the possibility of de novo species identification and verification using hexamer frequencies in combination with k-me ...[more]
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