Ontology highlight
ABSTRACT:
SUBMITTER: Muddana HS
PROVIDER: S-EPMC2832858 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20100201 7
We show that diffusion of single urease enzyme molecules increases in the presence of urea in a concentration-dependent manner and calculate the force responsible for this increase. Urease diffusion measured using fluorescence correlation spectroscopy increased by 16-28% over buffer controls at urea concentrations ranging from 0.001 to 1 M. This increase was significantly attenuated when urease was inhibited with pyrocatechol, demonstrating that the increase in diffusion was the result of enzyme ...[more]