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Mass spectrometric imaging of highly curved membranes during Tetrahymena mating.

ABSTRACT: Biological membrane fusion is crucial to numerous cellular events, including sexual reproduction and exocytosis. Here, mass spectrometry images demonstrate that the low-curvature lipid phosphatidylcholine is diminished in the membrane regions between fusing Tetrahymena, where a multitude of highly curved fusion pores exist. Additionally, mass spectra and principal component analysis indicate that the fusion region contains elevated amounts of 2-aminoethylphosphonolipid, a high-curvature lipid. This evidence suggests that biological fusion involves and might in fact be driven by a heterogeneous redistribution of lipids at the fusion site.

SUBMITTER: Ostrowski SG

PROVIDER: S-EPMC2833272 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature

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Mass spectrometric imaging of highly curved membranes during Tetrahymena mating.

Ostrowski Sara G SG Van Bell Craig T CT Winograd Nicholas N Ewing Andrew G AG

Science (New York, N.Y.) 20040701 5680

Biological membrane fusion is crucial to numerous cellular events, including sexual reproduction and exocytosis. Here, mass spectrometry images demonstrate that the low-curvature lipid phosphatidylcholine is diminished in the membrane regions between fusing Tetrahymena, where a multitude of highly curved fusion pores exist. Additionally, mass spectra and principal component analysis indicate that the fusion region contains elevated amounts of 2-aminoethylphosphonolipid, a high-curvature lipid. T ...[more]

PMID: 15232100

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