Ontology highlight
ABSTRACT:
SUBMITTER: Zaitsev S
PROVIDER: S-EPMC2835436 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Zaitsev Sergei S Spitzer Dirk D Murciano Juan-Carlos JC Ding Bi-Sen BS Tliba Samira S Kowalska M Anna MA Bdeir Khalil K Kuo Alice A Stepanova Victoria V Atkinson John P JP Poncz Mortimer M Cines Douglas B DB Muzykantov Vladimir R VR
The Journal of pharmacology and experimental therapeutics 20091201 3
Chemical coupling to carrier red blood cells (RBCs) converts tissue type plasminogen activator (tPA) from a problematic therapeutic into a safe agent for thromboprophylaxis. The goal of this study was to develop a more clinically relevant recombinant biotherapeutic by fusing a mutant tPA with a single-chain antibody fragment (scFv) with specificity for glycophorin A (GPA) on mouse RBCs. The fusion construct (anti-GPA scFv/PA) bound specifically to mouse but not human RBCs and activated plasminog ...[more]