Microbial community analysis of anodes from sediment microbial fuel cells powered by rhizodeposits of living rice plants.
Ontology highlight
ABSTRACT:
SUBMITTER: De Schamphelaire L
PROVIDER: S-EPMC2838000 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Publications
Microbial community analysis of anodes from sediment microbial fuel cells powered by rhizodeposits of living rice plants.
De Schamphelaire Liesje L Cabezas Angela A Marzorati Massimo M Friedrich Michael W MW Boon Nico N Verstraete Willy W
Applied and environmental microbiology 20100122 6
By placing the anode of a sediment microbial fuel cell (SMFC) in the rhizosphere of a rice plant, root-excreted rhizodeposits can be microbially oxidized with concomitant current generation. Here, various molecular techniques were used to characterize the composition of bacterial and archaeal communities on such anodes, as influenced by electrical circuitry, sediment matrix, and the presence of plants. Closed-circuit anodes in potting soil were enriched with Desulfobulbus-like species, members o ...[more]
Similar Datasets
Project description:Sediment microbial fuel cells (MFCs) were developed in which the complex substrates present in the sediment could be oxidized by microbes for electron production. In this study, the functional prediction of microbial communities of anode-associated soils in sediment MFCs was investigated based on 16S rRNA genes. Four computational approaches, including BugBase, Functional Annotation of Prokaryotic Taxa (FAPROTAX), the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2), and Tax4Fun2, were applied. A total of 67, 9, 37, and 38 functional features were statistically significant. Among these functional groups, the function related to the generation of precursor metabolites and energy was the only one included in all four computational methods, and the sum total of the proportion was 93.54%. The metabolism of cofactor, carrier, and vitamin biosynthesis was included in the three methods, and the sum total of the proportion was 29.94%. The results suggested that the microbial communities usually contribute to energy metabolism, or the metabolism of cofactor, carrier, and vitamin biosynthesis might reveal the functional status in the anode of sediment MFCs.
| S-EPMC9951962 | biostudies-literature
Project description:BackgroundThe flame-oxidized stainless steel anode (FO-SSA) is a newly developed electrode that enhances microbial fuel cell (MFC) power generation; however, substrate preference and community structure of the biofilm developed on FO-SSA have not been well characterized. Herein, we investigated the community on FO-SSA using high-throughput sequencing of the 16S rRNA gene fragment in acetate-, starch-, glucose-, and livestock wastewater-fed MFCs. Furthermore, to analyze the effect of the anode material, the acetate-fed community formed on a common carbon-based electrode-carbon-cloth anode (CCA)-was examined for comparison.ResultsSubstrate type influenced the power output of MFCs using FO-SSA; the highest electricity was generated using acetate as a substrate, followed by peptone, starch and glucose, and wastewater. Intensity of power generation using FO-SSA was related to the abundance of exoelectrogenic genera, namely Geobacter and Desulfuromonas, of the phylum Proteobacteria, which were detected at a higher frequency in acetate-fed communities than in communities fed with other substrates. Lactic acid bacteria (LAB)-Enterococcus and Carnobacterium-were predominant in starch- and glucose-fed communities, respectively. In the wastewater-fed community, members of phylum Planctomycetes were frequently detected (36.2%). Exoelectrogenic genera Geobacter and Desulfuromonas were also detected in glucose-, starch-, and wastewater-fed communities on FO-SSA, but with low frequency (0-3.2%); the lactate produced by Carnobacterium and Enterococcus in glucose- and starch-fed communities might affect exoelectrogenic bacterial growth, resulting in low power output by MFCs fed with these substrates. Furthermore, in the acetate-fed community on FO-SSA, Desulfuromonas was abundant (15.4%) and Geobacter had a minor proportion (0.7%), while in that on CCA, both Geobacter and Desulfuromonas were observed at similar frequencies (6.0-9.8%), indicating that anode material affects exoelectrogenic genus enrichment in anodic biofilm.ConclusionsAnodic community structure was dependent on both substrate and anode material. Although Desulfuromonas spp. are marine microorganisms, they were abundant in the acetate-fed community on FO-SSA, implying the presence of novel non-halophilic and exoelectrogenic species in this genus. Power generation using FO-SSA was positively related to the frequency of exoelectrogenic genera in the anodic community. Predominant LAB in saccharide-fed anodic biofilm caused low abundance of exoelectrogenic genera and consequent low power generation.
| S-EPMC5492815 | biostudies-literature
Project description:Sediment microbial fuel cells (SMFCs) have been used as renewable power sources for sensors in fresh and ocean waters. Organic compounds at the anode drive anodic reactions, while oxygen drives cathodic reactions. An understanding of oxygen reduction kinetics and the factors that determine graphite cathode performance is needed to predict cathodic current and potential losses, and eventually to estimate the power production of SMFCs. Our goals were to (1) experimentally quantify the dependence of oxygen reduction kinetics on temperature, electrode potential, and dissolved oxygen concentration for the graphite cathodes of SMFCs and (2) develop a mechanistic model. To accomplish this, we monitored current on polarized cathodes in river and ocean SMFCs. We found that (1) after oxygen reduction is initiated, the current density is linearly dependent on polarization potential for both SMFC types; (2) current density magnitude increases linearly with temperature in river SMFCs but remains constant with temperature in ocean SMFCs; (3) the standard heterogeneous rate constant controls the current density temperature dependence; (4) river and ocean SMFC graphite cathodes have large potential losses, estimated by the model to be 470 mV and 614 mV, respectively; and (5) the electrochemical potential available at the cathode is the primary factor controlling reduction kinetic rates. The mechanistic model based on thermodynamic and electrochemical principles successfully fit and predicted the data. The data, experimental system, and model can be used in future studies to guide SMFC design and deployment, assess SMFC current production, test cathode material performance, and predict cathode contamination.
| S-EPMC3551589 | biostudies-literature
Project description:Hygienic measures are extremely important to avoid the transmission of contagious viruses and diseases. The use of an electronic faucet increases the hygiene, encourages hand washing, avoids touching the faucet for opening and closing, and it saves water, since the faucet is automatically closed. The microbial fuel cell (MFC) technology has the capability to convert environmental waste into energy. The implementation of low cost ceramic MFCs into electronic interfaces integrated in toilets, would offer a compact powering system as well as an environmentally friendly small-scale treatment plant. In this work, the use of low cost ceramic MFCs to power an L20-E electronic faucet is presented for the first time. A single MFC was capable of powering an electronic faucet with an open/close cycle of 8.5 min, with 200 ml of urine. With a footprint of 360 cm3, the MFC could easily be integrated in a toilet. The possibility to power e-toilet components with MFCs offers a sustainable energy generation system. Other electronic components including an automatic flush, could potentially be powered by MFCs and contribute to the maintenance efficiency and hygiene of the public toilets, leading to a new generation of self-sustained energy recovering e-toilets.
| S-EPMC8363936 | biostudies-literature
Project description:The microbial community in the rhizosphere environment is critical for the health of land plants and the processing of soil organic matter. The objective of this study was to determine the extent to which rice plants shape the microbial community in rice field soil over the course of a growing season. Rice (Oryza sativa) was cultivated under greenhouse conditions in rice field soil from Vercelli, Italy and the microbial community in the rhizosphere of planted soil microcosms was characterized at four plant growth stages using quantitative PCR and 16S rRNA gene pyrotag analysis and compared to that of unplanted bulk soil. The abundances of 16S rRNA genes in the rice rhizosphere were on average twice that of unplanted bulk soil, indicating a stimulation of microbial growth in the rhizosphere. Soil environment type (i.e., rhizosphere versus bulk soil) had a greater effect on the community structure than did time (e.g., plant growth stage). Numerous phyla were affected by the presence of rice plants, but the strongest effects were observed for Gemmatimonadetes, Proteobacteria, and Verrucomicrobia. With respect to functional groups of microorganisms, potential iron reducers (e.g., Geobacter, Anaeromyxobacter) and fermenters (e.g., Clostridiaceae, Opitutaceae) were notably enriched in the rhizosphere environment. A Herbaspirillum species was always more abundant in the rhizosphere than bulk soil and was enriched in the rhizosphere during the early stage of plant growth.
| S-EPMC4710755 | biostudies-literature
Project description:BACKGROUND:Rice bran is a by-product of the rice milling process and mostly discarded in Japan. Although many studies have shown that microbial fuel cells (MFCs) are able to generate electricity from organic wastes, limited studies have examined MFCs for generating electricity from rice bran. FINDINGS:Laboratory-scale single-chamber MFCs were inoculated with paddy field soil and supplied with rice bran for examining electricity generation. Power outputs and microbiome compositions were compared between MFCs containing pure water as the liquid phase (MFC-W) and those containing mineral solution (MFC-M). Polarization analyses showed that both MFCs successfully generated electricity with the maximum power densities of 360 and 520 mW m-2 (based on the projected area of anode) for MFC-W and MFC-M, respectively. Amplicon-sequencing analyses revealed that Trichococcus and Geobacter specifically occurred in anode biofilms in MFC-W and MFC-M, respectively. CONCLUSIONS:The results suggest that rice bran is a feasible fuel by itself for generating electricity in MFCs.
| S-EPMC5120057 | biostudies-literature
Project description:Microbial fuel cells (MFCs) exploit the ability of microorganisms to generate electrical power during metabolism of substrates. However, the low efficiency of extracellular electron transfer from cells to the anode and the use of expensive rare metals as catalysts, such as platinum, limit their application and scalability. In this study we investigate the use of pristine graphene based electrodes at both the anode and the cathode of a MFC for efficient electrical energy production from the metabolically versatile bacterium Rhodopseudomonas palustris CGA009. We achieve a volumetric peak power output (PV) of up to 3.51 ± 0.50 W m-3 using graphene based aerogel anodes with a surface area of 8.2 m2 g-1. We demonstrate that enhanced MFC output arises from the interplay of the improved surface area, enhanced conductivity, and catalytic surface groups of the graphene based electrode. In addition, we show a 500-fold increase in PV to 1.3 ± 0.23 W m-3 when using a graphene coated stainless steel (SS) air cathode, compared to an uncoated SS cathode, demonstrating the feasibility of a platinum-free, graphene catalysed MFCs. Finally, we show a direct application for microwatt-consuming electronics by connecting several of these coin sized devices in series to power a digital clock.
| S-EPMC5795293 | biostudies-literature
Project description:Electricity generation from wheat straw hydrolysate and the microbial ecology of electricity-producing microbial communities developed in two-chamber microbial fuel cells (MFCs) were investigated. The power density reached 123 mW/m(2) with an initial hydrolysate concentration of 1,000 mg chemical oxygen demand (COD)/liter, while coulombic efficiencies ranged from 37.1 to 15.5%, corresponding to the initial hydrolysate concentrations of 250 to 2,000 mg COD/liter. The suspended bacteria found were different from the bacteria immobilized in the biofilm, and they played different roles in electricity generation from the hydrolysate. The bacteria in the biofilm were consortia with sequences similar to those of Bacteroidetes (40% of sequences), Alphaproteobacteria (20%), Bacillus (20%), Deltaproteobacteria (10%), and Gammaproteobacteria (10%), while the suspended consortia were predominately Bacillus (22.2%). The results of this study can contribute to improving understanding of and optimizing electricity generation in microbial fuel cells.
| S-EPMC2687294 | biostudies-literature
Project description:BACKGROUND: The microbial fuel cell represents a novel technology to simultaneously generate electric power and treat wastewater. Both pure organic matter and real wastewater can be used as fuel to generate electric power and the substrate type can influence the microbial community structure. In the present study, rice straw, an important feedstock source in the world, was used as fuel after pretreatment with diluted acid method for a microbial fuel cell to obtain electric power. Moreover, the microbial community structures of anodic and cathodic biofilm and planktonic culturewere analyzed and compared to reveal the effect of niche on microbial community structure. RESULTS: The microbial fuel cell produced a maximum power density of 137.6?±?15.5 mW/m2 at a COD concentration of 400 mg/L, which was further increased to 293.33?±?7.89 mW/m2 through adjusting the electrolyte conductivity from 5.6 mS/cm to 17 mS/cm. Microbial community analysis showed reduction of the microbial diversities of the anodic biofilm and planktonic culture, whereas diversity of the cathodic biofilm was increased. Planktonic microbial communities were clustered closer to the anodic microbial communities compared to the cathodic biofilm. The differentiation in microbial community structure of the samples was caused by minor portion of the genus. The three samples shared the same predominant phylum of Proteobacteria. The abundance of exoelectrogenic genus was increased with Desulfobulbus as the shared most abundant genus; while the most abundant exoelectrogenic genus of Clostridium in the inoculum was reduced. Sulfate reducing bacteria accounted for large relative abundance in all the samples, whereas the relative abundance varied in different samples. CONCLUSION: The results demonstrated that rice straw hydrolysate can be used as fuel for microbial fuel cells; microbial community structure differentiated depending on niches after microbial fuel cell operation; exoelectrogens were enriched; sulfate from rice straw hydrolysate might be responsible for the large relative abundance of sulfate reducing bacteria.
| S-EPMC3896841 | biostudies-other
Project description:The industrial contamination of marine sediments with mercury, silver, and zinc in Penang, Malaysia was studied with bio-remediation coupled with power generation using membrane less open (aerated) and closed (non-aerated) sediment microbial fuel cells (SMFCs). The prototype for this SMFC is very similar to a natural aquatic environment because it is not stimulated externally and an oxygen sparger is inserted in the cathode chamber to create the aerobic environment in the open SMFC and no oxygen supplied in the closed SMFC. The open and closed SMFCs were showed the maximum voltage generation 300.5 mV (77.75 mW/m2) and 202.7 mV (45.04 (mW/m2), respectively. The cyclic voltammetry showed the oxidation peak in open SMFCs at +1.9 μA and reduction peak at -0.3 μA but in closed SMFCs oxidation and reduction peaks were noted at +1.5 μA and -1.0 μA, respectively. The overall impedance (anode, cathode and solution) of closed SMFCs was higher than open SMFCs. The charge transfer impedance showed that the rates of substrate oxidation and reduction were very low in the closed SMFCs than open SMFCs. The Nyquist arc indicated that O2 act as electron acceptor in the open SMFCs and CO2 in the closed SMFCs. The highest remediation efficiency of toxic metals [Hg (II) ions, Zn (II) ions, and Ag (I) ions] in the open SMFCs were 95.03%, 86.69%, and 83.65% in closed SMFCs were 69.53%, 66.57%, and 65.33%, respectively, observed during 60-80 days. The scanning electron microscope and 16S rRNA analysis showed diverse exoelectrogenic community in the open SMFCs and closed SMFCs. The results demonstrated that open SMFCs could be employed for the power generation and bioremediation of pollutants.
| S-EPMC6339898 | biostudies-literature