Identifying the amylome, proteins capable of forming amyloid-like fibrils.

ABSTRACT: The amylome is the universe of proteins that are capable of forming amyloid-like fibrils. Here we investigate the factors that enable a protein to belong to the amylome. A major factor is the presence in the protein of a segment that can form a tightly complementary interface with an identical segment, which permits the formation of a steric zipper-two self-complementary beta sheets that form the spine of an amyloid fibril. Another factor is sufficient conformational freedom of the self-complementary segment to interact with other molecules. Using RNase A as a model system, we validate our fibrillogenic predictions by the 3D profile method based on the crystal structure of NNQQNY and demonstrate that a specific residue order is required for fiber formation. Our genome-wide analysis reveale

SUBMITTER: Goldschmidt L 

PROVIDER: S-EPMC2840437 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature