X chromosome-wide analyses of genomic DNA methylation states and gene expression in male and female neutrophils.

ABSTRACT: The DNA methylation status of human X chromosomes from male and female neutrophils was identified by high-throughput sequencing of HpaII and MspI digested fragments. In the intergenic and intragenic regions on the X chromosome, the sites outside CpG islands were heavily hypermethylated to the same degree in both genders. Nearly half of X chromosome promoters were either hypomethylated or hypermethylated in both females and males. Nearly one third of X chromosome promoters were a mixture of hypomethylated and heterogeneously methylated sites in females and were hypomethylated in males. Thus, a large fraction of genes that are silenced on the inactive X chromosome are hypomethylated in their promoter regions. These genes frequently belong to the evolutionarily younger strata of the X chromos

SUBMITTER: Yasukochi Y 

PROVIDER: S-EPMC2840519 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature