Ontology highlight
ABSTRACT:
SUBMITTER: Lokesh B
PROVIDER: S-EPMC2841200 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Lokesh Bhushan B Rashmi Panigrahi R PR Amruta Bhat S BS Srisathiyanarayanan Dharmaiah D Murthy Mathur R N MR Savithri Handanahal S HS
PloS one 20100318 3
Groundnut bud necrosis virus (GBNV), a member of genus Tospovirus in the family Bunyaviridae, infects a large number of leguminosae and solanaceae plants in India. With a view to elucidate the function of nonstructural protein, NSs encoded by the small RNA genome (S RNA), the NSs protein of GBNV- tomato (Karnataka) was over-expressed in E. coli and purified by Ni-NTA chromatography. The purified rNSs protein exhibited an RNA stimulated NTPase activity. Further, this activity was metal ion depend ...[more]