LC/MS analysis of cellular RNA reveals NAD-linked RNA.
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ABSTRACT:
SUBMITTER: Chen YG
PROVIDER: S-EPMC2842606 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Publications
LC/MS analysis of cellular RNA reveals NAD-linked RNA.
Chen Y Grace YG Kowtoniuk Walter E WE Agarwal Isha I Shen Yinghua Y Liu David R DR
Nature chemical biology 20091011 12
We developed a general method to detect cellular small molecule-RNA conjugates that does not rely on the reactivity of the small molecule. This technique revealed NAD-linked RNA in Escherichia coli and Streptomyces venezuelae. Subsequent characterization showed that NAD is a 5' modification of RNA, cannot be installed in vitro through aberrant transcriptional initiation, is only found among smaller cellular RNAs and is present at a surprisingly high abundance of approximately 3,000 copies per ce ...[more]
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