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Fluorescent structural DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates.

ABSTRACT: Highly labeled DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates (dNTPs) were developed as a source of dNTPs for DNA polymerase. The particles were prepared by strand-displacement polymerization from a self-complementary circular template. Imaged by atomic force microscopy, these functionalized particles appear as condensed fuzzy balls with diameters between 50 and 150 nm. They emit a bright fluorescent signal, detected in 2 ms exposures with a signal-to-noise ratio of 25 when imaged using a TIR fluorescence microscope.

SUBMITTER: Anderson JP

PROVIDER: S-EPMC2843431 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature

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Fluorescent structural DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates.

Anderson Jon P JP Reynolds Bambi L BL Baum Kristin K Williams John G JG

Nano letters 20100301 3

Highly labeled DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates (dNTPs) were developed as a source of dNTPs for DNA polymerase. The particles were prepared by strand-displacement polymerization from a self-complementary circular template. Imaged by atomic force microscopy, these functionalized particles appear as condensed fuzzy balls with diameters between 50 and 150 nm. They emit a bright fluorescent signal, detected in 2 ms exposures with a signal-to-noise ratio of ...[more]

PMID: 20158249

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