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Nitrile-inducible gene expression in mycobacteria.


ABSTRACT: The ability to ectopically control gene expression is a fundamental tool for the study of bacterial physiology and pathogenesis. While many efficient inducible expression systems are available for Gram-negative bacteria, few are useful in phylogenetically distant organisms, such as mycobacteria. We have adapted a highly-inducible regulon of Rhodococcus rhodochrous to artificially regulate gene expression in both rapidly-growing environmental mycobacteria and slow-growing pathogens, such as Mycobacterium tuberculosis. We demonstrate that this artificial regulatory circuit behaves as a bistable switch, which can be manipulated regardless of growth phase in vitro, and during intracellular growth in macrophages. High-level overexpression is also possible, facilitating biochemical and structural studies of mycobacterial proteins produced in their native host.

SUBMITTER: Pandey AK 

PROVIDER: S-EPMC2845969 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature

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Nitrile-inducible gene expression in mycobacteria.

Pandey Amit K AK   Raman Sahadevan S   Proff Rose R   Joshi Swati S   Kang Choong-Min CM   Rubin Eric J EJ   Husson Robert N RN   Sassetti Christopher M CM  

Tuberculosis (Edinburgh, Scotland) 20080917 1


The ability to ectopically control gene expression is a fundamental tool for the study of bacterial physiology and pathogenesis. While many efficient inducible expression systems are available for Gram-negative bacteria, few are useful in phylogenetically distant organisms, such as mycobacteria. We have adapted a highly-inducible regulon of Rhodococcus rhodochrous to artificially regulate gene expression in both rapidly-growing environmental mycobacteria and slow-growing pathogens, such as Mycob  ...[more]

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