Ontology highlight
ABSTRACT:
SUBMITTER: Dragset MS
PROVIDER: S-EPMC4562662 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Dragset Marte S MS Barczak Amy K AK Kannan Nisha N Mærk Mali M Flo Trude H TH Valla Svein S Rubin Eric J EJ Steigedal Magnus M
PloS one 20150908 9
Conditional expression is a powerful tool to investigate the role of bacterial genes. Here, we adapt the Pseudomonas putida-derived positively regulated XylS/Pm expression system to control inducible gene expression in Mycobacterium smegmatis and Mycobacterium tuberculosis, the causative agent of human tuberculosis. By making simple changes to a Gram-negative broad-host-range XylS/Pm-regulated gene expression vector, we prove that it is possible to adapt this well-studied expression system to no ...[more]