Ontology highlight
ABSTRACT:
SUBMITTER: Gu J
PROVIDER: S-EPMC2847011 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Gu Jiafeng J Li Sicong S Zhang Xiaoshan X Wang Ling-Chi LC Niewolik Doris D Schwarz Klaus K Legerski Randy J RJ Zandi Ebrahim E Lieber Michael R MR
DNA repair 20100201 4
Human nuclease Artemis belongs to the metallo-beta-lactamase protein family. It acquires double-stranded DNA endonuclease activity in the presence of DNA-PKcs. This double-stranded DNA endonuclease activity is critical for opening DNA hairpins in V(D)J recombination and is thought to be important for processing overhangs during the nonhomologous DNA end joining (NHEJ) process. Here we show that purified human Artemis exhibits single-stranded DNA endonuclease activity. This activity is proportion ...[more]