Chemical-biological fingerprinting: probing the properties of DNA lesions formed by peroxynitrite.

ABSTRACT: DNA-damaging agents usually produce a vast collection of lesions within the genome. Analysis of these lesions from the structural and biological viewpoints is often complicated by the reality that some of the lesions are chemically fragile, leading to an even larger set of secondary and tertiary products. In an effort to deconvolute complex DNA-damage spectra, a strategy is presented whereby an oligonucleotide containing a specific target for chemical reaction is allowed to react with a DNA-damaging agent. A large collection of HPLC-resolvable modified oligonucleotides is generated, and chromatographically distinct members of the set are then individually characterized using chemical, spectroscopic, biochemical, and genetic probes. The biological component of this "chemical-biological fing

SUBMITTER: Delaney S 

PROVIDER: S-EPMC2848752 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature