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Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions.

ABSTRACT: We describe a Toxoplasma gondii strain that will permit the use of site-specific recombination to study the host-parasite interactions of this organism. This Toxoplasma strain efficiently injects a Cre fusion protein into host cells. In a Cre-reporter cell line, a single parasite invasion induced Cre-mediated recombination in 95% of infected host cells. By infecting Cre-reporter mice with these parasites, we also monitored host-cell infection in vivo.

SUBMITTER: Koshy AA

PROVIDER: S-EPMC2850821 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature

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Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions.

Koshy Anita A AA Fouts Ashley E AE Lodoen Melissa B MB Alkan Ozan O Blau Helen M HM Boothroyd John C JC

Nature methods 20100307 4

We describe a Toxoplasma gondii strain that will permit the use of site-specific recombination to study the host-parasite interactions of this organism. This Toxoplasma strain efficiently injects a Cre fusion protein into host cells. In a Cre-reporter cell line, a single parasite invasion induced Cre-mediated recombination in 95% of infected host cells. By infecting Cre-reporter mice with these parasites, we also monitored host-cell infection in vivo. ...[more]

PMID: 20208532

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