Ontology highlight
ABSTRACT:
SUBMITTER: Kingery DA
PROVIDER: S-EPMC2851197 | biostudies-literature | 2008 May
REPOSITORIES: biostudies-literature
Kingery David A DA Pfund Emmanuel E Voorhees Rebecca M RM Okuda Kensuke K Wohlgemuth Ingo I Kitchen David E DE Rodnina Marina V MV Strobel Scott A SA
Chemistry & biology 20080501 5
The ribosome has an active site comprised of RNA that catalyzes peptide bond formation. To understand how RNA promotes this reaction requires a detailed understanding of the chemical transition state. Here, we report the Brønsted coefficient of the alpha-amino nucleophile with a series of puromycin derivatives. Both 50S subunit- and 70S ribosome-catalyzed reactions displayed linear free-energy relationships with slopes close to zero under conditions where chemistry is rate limiting. These result ...[more]