Ontology highlight
ABSTRACT:
SUBMITTER: DiMattia MA
PROVIDER: S-EPMC2851902 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
DiMattia Michael A MA Watts Norman R NR Stahl Stephen J SJ Rader Christoph C Wingfield Paul T PT Stuart David I DI Steven Alasdair C AC Grimes Jonathan M JM
Proceedings of the National Academy of Sciences of the United States of America 20100315 13
HIV-1 Rev is a small regulatory protein that mediates the nuclear export of viral mRNAs, an essential step in the HIV replication cycle. In this process Rev oligomerizes in association with a highly structured RNA motif, the Rev response element. Crystallographic studies of Rev have been hampered by the protein's tendency to aggregate, but Rev has now been found to form a stable soluble equimolar complex with a specifically engineered monoclonal Fab fragment. We have determined the structure of ...[more]