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Metal-Containing Polystyrene Beads as Standards for Mass Cytometry.

ABSTRACT: We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions within the interior of the beads. Mass cytometry enabled the bead-by-bead measurement of the metal-content and determination of the metal-content distribution. Beads synthesized by dispersion polymerization that involved three stages were shown to have narrower bead-to-bead variation in their lanthanide content than beads synthesized by 2-stage dispersion polymerization. The beads exhibited insignificant release of their lanthanide content to aqueous solutions of different pHs over a period of six months. When mixed with KG1a or U937 cell lines, metal-containing polymer beads were shown not to affect the mass cytometry response to the metal content of element-tagged antibodies specifically attached to these cells.

SUBMITTER: Abdelrahman AI

PROVIDER: S-EPMC2852877 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature

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Metal-Containing Polystyrene Beads as Standards for Mass Cytometry.

Abdelrahman Ahmed I AI Ornatsky Olga O Bandura Dmitry D Baranov Vladimir V Kinach Robert R Dai Sheng S Thickett Stuart C SC Tanner Scott S Winnik Mitchell A MA

Journal of analytical atomic spectrometry 20100101 3

We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions ...[more]

PMID: 20390041

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Metal-Containing Polystyrene Beads as Standards for Mass Cytometry.

Publications

Metal-Containing Polystyrene Beads as Standards for Mass Cytometry.

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