Normalization of mass cytometry data with bead standards.
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ABSTRACT:
SUBMITTER: Finck R
PROVIDER: S-EPMC3688049 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Publications
Normalization of mass cytometry data with bead standards.
Finck Rachel R Simonds Erin F EF Jager Astraea A Krishnaswamy Smita S Sachs Karen K Fantl Wendy W Pe'er Dana D Nolan Garry P GP Bendall Sean C SC
Cytometry. Part A : the journal of the International Society for Analytical Cytology 20130319 5
Mass cytometry uses atomic mass spectrometry combined with isotopically pure reporter elements to currently measure as many as 40 parameters per single cell. As with any quantitative technology, there is a fundamental need for quality assurance and normalization protocols. In the case of mass cytometry, the signal variation over time due to changes in instrument performance combined with intervals between scheduled maintenance must be accounted for and then normalized. Here, samples were mixed w ...[more]
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