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Engineering of Streptomyces platensis MA7339 for overproduction of platencin and congeners.

ABSTRACT: Platensimycin (1) and platencin (2) are novel antibiotic leads against multidrug resistant pathogens. The production of 2 in Streptomyces platensis MA7339 is under the control of ptnR1, a GntR-like transcriptional regulator. Inactivating ptnR1 afforded S. platensis MA7339 mutant strain SB12600 that overproduces 2 at a titer approximately 100-fold greater than that from the wild-type strain and accumulates platencin A(1) (3) and eight new congeners, platencins A(2)-A(9) (4-11). The isolation, structural elucidation, and antibacterial activity of 4-11, in comparison to 1-3, are described.

SUBMITTER: Yu Z

PROVIDER: S-EPMC2855538 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature

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Engineering of Streptomyces platensis MA7339 for overproduction of platencin and congeners.

Yu Zhiguo Z Smanski Michael J MJ Peterson Ryan M RM Marchillo Karen K Andes David D Rajski Scott R SR Shen Ben B

Organic letters 20100401 8

Platensimycin (1) and platencin (2) are novel antibiotic leads against multidrug resistant pathogens. The production of 2 in Streptomyces platensis MA7339 is under the control of ptnR1, a GntR-like transcriptional regulator. Inactivating ptnR1 afforded S. platensis MA7339 mutant strain SB12600 that overproduces 2 at a titer approximately 100-fold greater than that from the wild-type strain and accumulates platencin A(1) (3) and eight new congeners, platencins A(2)-A(9) (4-11). The isolation, str ...[more]

PMID: 20232845

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