Ontology highlight
ABSTRACT:
SUBMITTER: Neumann H
PROVIDER: S-EPMC2856916 | biostudies-literature | 2009 Oct
REPOSITORIES: biostudies-literature
Neumann Heinz H Hancock Susan M SM Buning Ruth R Routh Andrew A Chapman Lynda L Somers Joanna J Owen-Hughes Tom T van Noort John J Rhodes Daniela D Chin Jason W JW
Molecular cell 20091001 1
Lysine acetylation of histones defines the epigenetic status of human embryonic stem cells and orchestrates DNA replication, chromosome condensation, transcription, telomeric silencing, and DNA repair. A detailed mechanistic explanation of these phenomena is impeded by the limited availability of homogeneously acetylated histones. We report a general method for the production of homogeneously and site-specifically acetylated recombinant histones by genetically encoding acetyl-lysine. We reconsti ...[more]