Ontology highlight
ABSTRACT:
SUBMITTER: Flynn EM
PROVIDER: S-EPMC2858566 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Flynn E Megan EM Hanson Jeffrey A JA Alber Tom T Yang Haw H
Journal of the American Chemical Society 20100401 13
The Mycobacterium tuberculosis protein tyrosine phosphatase PtpB shows resistance to the oxidative conditions that prevail within an infected host macrophage, but the mechanism of this molecular adaptation is unknown. Crystal structures of PtpB revealed previously that a closed, two-helix lid covers the active site. By measuring single-molecule Forster-type resonance energy transfer to probe the dynamics of two helices that constitute the lid, we obtained direct evidence for large, spontaneous o ...[more]