Ontology highlight
ABSTRACT:
SUBMITTER: Thekisoe OM
PROVIDER: S-EPMC2861400 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Thekisoe Oriel M M OM Rodriguez Carol V CV Rivas Francisco F Coronel-Servian Andrea M AM Fukumoto Shinya S Sugimoto Chihiro C Kawazu Shin-Ichiro S Inoue Noboru N
The American journal of tropical medicine and hygiene 20100501 5
We have developed two loop-mediated isothermal amplification (LAMP) assays for specific detection of Trypanosoma cruzi and Trypanosoma rangeli based on the 18S ribosomal RNA (rRNA) and the small nucleolar RNA (snoRNA) genes, respectively. The detection limit of the assays is 100 fg and 1 pg for T. cruzi and T. rangeli, respectively, with reactions conducted in 60 minutes. The two LAMP assays were used in detection of T. cruzi and T. rangeli infections in comparison with polymerase chain reaction ...[more]