New compstatin variants through two de novo protein design frameworks.
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ABSTRACT:
SUBMITTER: Bellows ML
PROVIDER: S-EPMC2872270 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Publications
New compstatin variants through two de novo protein design frameworks.
Bellows M L ML Fung H K HK Taylor M S MS Taylor M S MS Floudas C A CA López de Victoria A A Morikis D D
Biophysical journal 20100501 10
Two de novo protein design frameworks are applied to the discovery of new compstatin variants. One is based on sequence selection and fold specificity, whereas the other approach is based on sequence selection and approximate binding affinity calculations. The proposed frameworks were applied to a complex of C3c with compstatin variant E1 and new variants with improved binding affinities are predicted and experimentally validated. The computational studies elucidated key positions in the sequenc ...[more]
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