Ontology highlight
ABSTRACT:
SUBMITTER: Wang J
PROVIDER: S-EPMC2873192 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
Wang Jinbu J Zuo Xiaobing X Yu Ping P Byeon In-Ja L IJ Jung Jinwon J Wang Xiaoxia X Dyba Marzena M Seifert Soenke S Schwieters Charles D CD Qin Jun J Gronenborn Angela M AM Wang Yun-Xing YX
Journal of the American Chemical Society 20090801 30
Determining architectures of multicomponent proteins or protein complexes in solution is a challenging problem. Here we report a methodology that simultaneously uses residual dipolar couplings (RDC) and the small-angle X-ray scattering (SAXS) restraints to mutually orient subunits and define the global shape of multicomponent proteins and protein complexes. Our methodology is implemented in an efficient algorithm and demonstrated using five examples. First, we demonstrate the general approach wi ...[more]