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Temporal pixel multiplexing for simultaneous high-speed, high-resolution imaging.


ABSTRACT: We introduce an imaging modality that, by offsetting pixel-exposure times during capture of a single image frame, embeds temporal information in each frame. This allows simultaneous acquisition of full-resolution images at native detector frame rates and high-speed image sequences at reduced resolution, without increasing bandwidth requirements. We demonstrate this method using macroscopic and microscopic examples, including imaging calcium transients in heart cells at 250 Hz using a 10-Hz megapixel camera.

SUBMITTER: Bub G 

PROVIDER: S-EPMC2873566 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature

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Temporal pixel multiplexing for simultaneous high-speed, high-resolution imaging.

Bub Gil G   Tecza Matthias M   Helmes Michiel M   Lee Peter P   Kohl Peter P  

Nature methods 20100214 3


We introduce an imaging modality that, by offsetting pixel-exposure times during capture of a single image frame, embeds temporal information in each frame. This allows simultaneous acquisition of full-resolution images at native detector frame rates and high-speed image sequences at reduced resolution, without increasing bandwidth requirements. We demonstrate this method using macroscopic and microscopic examples, including imaging calcium transients in heart cells at 250 Hz using a 10-Hz megap  ...[more]

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