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High-Speed Super-Resolution Imaging Using Protein-Assisted DNA-PAINT.

ABSTRACT: Super-resolution imaging allows for the visualization of cellular structures on a nanoscale level. DNA-PAINT (DNA point accumulation in nanoscale topology) is a super-resolution method that depends on the binding and unbinding of DNA imager strands. The current DNA-PAINT technique suffers from slow acquisition due to the low binding rate of the imager strands. Here we report on a method where imager strands are loaded into a protein, Argonaute (Ago), which allows for faster binding. Ago preorders the DNA imager strand into a helical conformation, allowing for 10 times faster target binding. Using a 2D DNA origami structure, we demonstrate that Ago-assisted DNA-PAINT (Ago-PAINT) can speed up the current DNA-PAINT technique by an order of magnitude, while maintaining the high spatial resolution. We envision this tool to be useful for super-resolution imaging and other techniques that rely on nucleic acid interactions.

SUBMITTER: Filius M

PROVIDER: S-EPMC7146856 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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High-Speed Super-Resolution Imaging Using Protein-Assisted DNA-PAINT.

Filius Mike M Cui Tao Ju TJ Ananth Adithya N AN Docter Margreet W MW Hegge Jorrit W JW van der Oost John J Joo Chirlmin C

Nano letters 20200320 4

Super-resolution imaging allows for the visualization of cellular structures on a nanoscale level. DNA-PAINT (DNA point accumulation in nanoscale topology) is a super-resolution method that depends on the binding and unbinding of DNA imager strands. The current DNA-PAINT technique suffers from slow acquisition due to the low binding rate of the imager strands. Here we report on a method where imager strands are loaded into a protein, Argonaute (Ago), which allows for faster binding. Ago preorder ...[more]

PMID: 32168456

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