Ontology highlight
ABSTRACT:
SUBMITTER: Chen PR
PROVIDER: S-EPMC2873846 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Angewandte Chemie (International ed. in English) 20090101 22
A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl-tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl-tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA-aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins. ...[more]