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A facile system for encoding unnatural amino acids in mammalian cells.

ABSTRACT: A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl-tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl-tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA-aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins.

SUBMITTER: Chen PR 

PROVIDER: S-EPMC2873846 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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A facile system for encoding unnatural amino acids in mammalian cells.

Chen Peng R PR   Groff Dan D   Guo Jiantao J   Ou Weijia W   Cellitti Susan S   Geierstanger Bernhard H BH   Schultz Peter G PG  

Angewandte Chemie (International ed. in English) 20090101 22

A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl-tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl-tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA-aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins. ...[more]

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