Ontology highlight
ABSTRACT:
SUBMITTER: Chatterjee A
PROVIDER: S-EPMC3718144 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Chatterjee Abhishek A Xiao Han H Bollong Michael M Ai Hui-Wang HW Schultz Peter G PG
Proceedings of the National Academy of Sciences of the United States of America 20130701 29
Here we report the development of a baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells. We have exploited the large cargo-capacity (>30 kb) and stability of the double-stranded DNA genome of baculovirus to deliver to a variety of cell types all of the components required to genetically incorporate novel amino acids. These include the engineered tRNA/aminoacyl-tRNA synthetase pair and the nonsense mutant of the targ ...[more]