Diffusion of the reaction boundary of rapidly interacting macromolecules in sedimentation velocity.

ABSTRACT: Sedimentation velocity analytical ultracentrifugation combines relatively high hydrodynamic resolution of macromolecular species with the ability to study macromolecular interactions, which has great potential for studying dynamically assembled multiprotein complexes. Complicated sedimentation boundary shapes appear in multicomponent mixtures when the timescale of the chemical reaction is short relative to the timescale of sedimentation. Although the Lamm partial differential equation rigorously predicts the evolution of concentration profiles for given reaction schemes and parameter sets, this approach is often not directly applicable to data analysis due to experimental and sample imperfections, and/or due to unknown reaction pathways. Recently, we have introduced the effective particle

SUBMITTER: Schuck P 

PROVIDER: S-EPMC2877318 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature